European Journal of Biomedical and Pharmaceutical Sciences

DETERMINATION OF QUERCETIN IN METHANOLIC EXTRACT OF SESBANIASESBAN (FABACEAE) BY HPTLC METHOD

Priyanka D. Kandhare and Padmanabh B. Deshpande*

ABSTRACT

Sesbania sesban has been used traditionally for different activities such as astringent, anti-inflammatory, purgative, demulcent and anthelmintic. Phytochemical analysis of the plant confirmed the presence of alkaloids, carbohydrates, proteins, phytosterol, phenol, flavonoids etc. In the current study, an effort was made to quantify the flavonoid quercetin in the stem extract. TLC was performed to confirm the presence of quercetin and HPTLC procedure has been developed for quantification of quercetin in the methanolic stem extract. HPTLC separation was carried out on Merck TLC aluminium sheets precoated with silica gel 60F254 using Toluene: Ethyl acetate: Formic acid: Methanol (5.5: 3: 1: 0.5, v/v/v/v) as mobile phase. Quantitative analysis was carried out in the absorbance at 293 nm. A good linear relationship was obtained in the concentration range of 200-1200 ng band-1.

Keywords: Sesbania sesban, anti-inflammatory, quercetin, TLC, HPTLC, Validation.