European Journal of Biomedical and Pharmaceutical Sciences

ISOLATION, SCREENING, CHARACTERIZATION AND DETECTION OF MULTIDRUG RESISTANCE IN POLYHYDROXY BUTYRATE PRODUCERS FROM DIFFERENT SOIL SAMPLES

Jasmine R.* and Hannah

ABSTRACT

Rapid accumulation of plastics in the environment affects wildlife, wildlife habitat and humans. Most of the animals can also be affected through entanglement, direct ingestion of plastic waste, or through exposure to chemicals within plastics that cause metabolic imbalances leading to metabolic changes. Hence, there is an urgent need for “eco-friendly” plastics. PHBs (Polyhydroxy butyrates) are good alternatives of synthetic plastics since they closely resemble synthetic plastics. Polyhydroxybutyrates (PHB) are biodegradable polyesters synthesized by many bacteria. Such bacteria were isolated and screened for PHB production by sudan black dye method. Also the antimicrobial activity of PHB producers was screened and the antibiogram was obtained. It was observed that among the bacterial isolates from three soil samples used in the present study, isolate 3 obtained from corn soil was able to produce maximum yield of PHB. Antibiotic profiling revealed a variation in the resistance pattern to the selected antibiotics. Among the three PHB producing bacteria isolated from soil, one isolate was found to be promising in PHB production and was also found to be multi drug resistant.

Keywords: Polyhydroxy Butyrate, Sudan Black, Antibiotic, Biodegradable.