European Journal of Biomedical and Pharmaceutical Sciences

PHARMACOPEIAL STANDARD DEVELOPMENT, HPTLC. FINGERPRINTING AND PHYSICOCHEMICAL RESEARCH STUDIES OF UNANI ANTI-PARALYTIC CLASSICAL DRUG RAUGHAN-E-HAFT BARG

*Pawan Kumar Sagar, Munawwar Husain Kazmi, Javed Inam Siddiqui, M. A. Rasheed N,

ABSTRACT

Pharmacopeial Standard development, HPTLC. Fingerprinting and Physicochemical research studies of Unani Anti-paralytic drug Raughan-e-Haft Barg , classical formulation used as local application in the treatment of Falij (Paralysis) and Laqwa (Facial Paralysis) and other Neurological disorders since ancient time.Three samples of the drug Raughan-e-Haft Barg taken into these studies which prepared at the Pharmacy of CRIUM. (Under CCRUM. Ministry of AYUSH.) Hyderabad by employing authenticated standard methods. The quality control & quality assurance studies were conducted in accordance to
the WHO., AOAC., IPC. and UPC., approved guidelines.The obtained physicochemical data showed that the drug samples contained Petroleum ether (60-80ï‚°C) Extractive, %,w/v. (84.52), Acid value (3.06), Iodine value (6.36), Peroxide value (8.78), Unsaponifiable matter(16.71),Refractive index (1.480),Weight per ml. (gm.)(0.91), Test for presence of Arachis oil, Cotton seed oil, Sesame, Mineral oil (all are negatives)were assessed in Raughan-e-Haft Barg. HPTLC. studies of aqueous and alcohol extracts of both formulation showed various spots at 366nm and 254nm (UV. region),under exposed to Iodine vapours obtained with equate, best separation using selected suitable solvent system of mobile phase. The quality control studies results revealed the absence of hazardous and toxic contamination from the drug sample. Moreover the obtained research studies data and comparative screening will provide the referential supportive information in the development of pharmacopeial standard monographs, identification, reinvestigation, quality assurance and pharmacovigilance of the drug.

Keywords: Raughan-e-Haft Barg, Anti-paralytic, development of pharmacopeial standards, physicochemical, comparative screening, quality control and quality assurance.