European Journal of Biomedical and Pharmaceutical Sciences

IN VITRO ANTIMICROBIAL ACTIVITY OF PUNICA GRANATUM, HIBISCUS SABDARIFFA AND ANNONA SQUAMOSA EXTRACTS AGAINST SOME MICROORGANISMS ISOLATED FROM WOMEN WITH HIGH VAGINAL INFECTIONS

Hammood Hussein, Buthainah M. Taha*, Sinaa Mahdi Shakir and Ban Mahdi Shakir

ABSTRACT

Through a period extended for three months (October to December, 2017), 25 fungal and bacterial isolates were isolated from Fattima-AL-Zahra hospital for pediatric and obstetric in Baghdad from women with high vaginal infections. Out of these 25 isolates, 16 (64%) were Candida albicans isolates, 4 (16%) were Escherichia coli isolates, 3 (12%) were Enterococcus faecalis isolates and 2 (8%) were Staphylococcus saprophyticus isolates. Escherichia coli isolates showed the highest resistant toward Ampicillin and Cefazolin which gave 4(100%) resistance rate. On the other hand the most effective antibiotic toward Escherichia coli isolates under test was Amikacin which it gave the lowest resistance rate 0(0%). Enterococcus faecalis and Staphylococcus saprophyticus isolates showed a high level resistance to most of the antibiotics under test and the highest resistant antibiotics were Ampicillin and Erythromycin which gave 5(100%) resistance rate, while the most effective antibiotic was Vancomycin which it gave 0 (0%) resistance rate. Punica granatum (peel), Hibiscus sabdariffa and Annona squamosa (peel and seed) extracts were prepared in different concentrations (100, 50, 25 and 12. 5 mg/ml). The result of antimicrobial activity test of Punica granatum (peel) extract showed that the Punica granatum extract was the most effective extract on fungal and bacterial isolates in all concentrations compared with the other plant extracts under study. On other hand Hibiscus sabdariffa extract was effective on fungal and bacterial isolates only in 100, 50 and 25 mg/ml concentrations and not gave antimicrobial activity in the last concentration. The result of antimicrobial activity test of Annona squamosa (peel and seed) extracts showed that Annona squamosa (peel and seed) extracts don’t gave antimicrobial activity against all fungal and bacterial isolates under test (G-ve and G+ve bacteria) in all concentrations (100, 50, 25 and 12.5 mg/ml concentrations).

Keywords: Antimicrobial activity, High vaginal swabs, Punica granatum, Hibiscus sabdariffa and Annona squamosal.