European Journal of Biomedical and Pharmaceutical Sciences

ANTI-TUMOR EFFECT OF GRAVIOLA AND/OR CRANBERRY AS A NATURAL ANTI-PROLIFERATIVE AGENT ON COLORECTAL CELL LINES (CACO-2)

Iman Hesham El-Khashab* and Nadia Noble Daoud Aniss

ABSTRACT

The purpose of this study was to examine the anti-tumor effect of Graviola and/ or Cranberry on colorectal cancer cell lines (Caco2). Graviola and/or Cranberry were obtained and tested in vitro for their anti-proliferative activity against the colorectal cell lines (Caco2) for 24h by the MTT cell viability assay. DNA fragmentation was assessed by gel electrophoresis. Trypan blue assay was performed to determine the viability of cells. DNA content and distribution in different stages of cells and cell death were measured by flow cytometry. The result of this study highlighted the anti-tumor effect of Graviola and or Cranberry on Caco2. Moreover IC50 concentration of Graviola alone or with Cranberry were able to inhibit more than half of the percentage of colorectal cancer cell lines indicating an interesting anti-proliferating activity of Graviola alone or with Cranberry. This study suggests that Graviola alone or with Cranberry could be used as a source of natural anti-proliferative agent and thus could be useful as anti-tumor agent against colorectal cancer cells and is worth further investigation.

Keywords: Colorectal cell lines- Graviola- Cranberry- anti-tumor – cell viability.