European Journal of Biomedical and Pharmaceutical Sciences

MOLECULAR DIAGNOSIS FOR SURFACE ANTIGENS GENES (SAG1, SAG2, & SAG3) OF TOXOPLASMOSIS IN BASRAH PROVINCE-IRAQ

Iltefat A. Al-Tamemmi*, Basim H. Abdullah and Shaymaa Jabbar Raisan

ABSTRACT

The current study was done in Basrah province during the period from January 2017 to September 2017. The results show that 8 (68.5%) were positive by conventional PCR for SAG1 gene and 12 (17.1%), 13 (25.7%) were positive by nested-PCR for SAG2, SAG3 genes respectively, whereas 4 (20%) of negative latex (control group) was positive by PCR for SAG1 gene only. No statistically significant difference between PCR positive for SAG1 and gender but there are significant role with age for same gene, also there are a relationship between infection with toxoplasmosis and time of abortion in aborted women, no positive case were seen in 3th trimester of pregnancy in all genes.

Keywords: Toxoplasma, surface antigen, SAG1, SAG2, SAG3, Basrah, Iraq.