European Journal of Biomedical and Pharmaceutical Sciences

THE EMERGING TREND OF BRUCELLA ABORTUS ZOONOTIC INCIDENCE IN BOTH HUMAN AND ANIMAL POPULATION OF DISTRICT SWAT KHYBER-PAKHTUNKHWA

Maria Saif*, Wajid Khan, Fakhur Ul Islam, Khurshaid Anwar, Insanuddin, Habibun Nabi, Ihsanuddin, Syed Ishfaq Ahmad and Hanif Ur Rahman*

ABSTRACT

Brucellosis is a common zoonotic disease caused by a member of the genus Brucella, which affects both animals and humans. The aim of the current study is to record the incidence of brucellosis among livestock engaged human and the comparative study of two Brucella species, Brucella abortus, and Brucella melitensis in animal and human population in District Swat. A total of two hundred blood samples (100 each from human and animals) were collected from 3 tehsils i.e. Kabal, Khwza Khela and Matta of District Swat. For the analysis of these samples, Standard Plate Agglutination Test (SPAT) and molecular detection were carried out. Out of the total samples, 23% for humans and 18% for animals were found positive on the SPAT test. For species identification, two sets of species-specific primers were used for Brucella abortus and Brucella melitensis and amplicons having a size of 498 bp and 731 bp were obtained respectively. Out of 200 samples, 11% were confirmed through PCR by detecting IS711 gene in the Brucella genome. The presence of Brucella abortus and Brucella melitensis was identified and confirmed through PCR in the studied area. This study demonstrates that brucellosis is the most prevalent zoonotic disease in district Swat. Our results showed that the zoonotic incidence of Brucella abortus infection is increasing in both animals and Human population of the studied area as compared to the Brucella melitensis infection.

Keywords: Brucella, Brucella abortus, Brucella melitensis, IS711, Human, Animals District Swat.