Abstract
DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPTLC METHOD FOR ESTIMATION OF DAPSONE

Santosh V. Gandhi* and Gaurav R. Patil

ABSTRACT

A simple, precise and sensitive stability indicating high performance thin layer chromatographic (HPTLC) method has been developed and validated for the analysis of Dapsone in bulk and in tablet dosage form. The separation was performed on pre-coated silica gel 60 GF254 plates using Toluene: Ethyl acetate (5:5 v/v) as mobile phase. The retention factor (Rf) was found to be 0.38. The detection of band was carried out at 295 nm.The drug was subjected to different stress conditions like acid, base hydrolysis, oxidation, thermal degradation and photolysis. The method was successfully validated according to ICH Q2 (R1) guidelines. The linear regression analysis data for the calibration plot showed good linear relationship with R2 =0.9534 in the range of 200-1200 ng band-1. The method found to be accurate as results of the recovery studies are close to the 100 %. The developed method can be adopted for routine analysis of Dapsone in bulk and pharmaceutical dosage form.

Keywords: High performance thin layer chromatography (HPTLC), Dapsone, method validation, stability indicating method.


[Full Text Article]   [Download Certificate]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Dr. Dhrubo Jyoti Sen

Download Article : Click here

News & Updation

  • EJBPS: APRIL ISSUE PUBLISHED

    APRIL 2024 Issue has been successfully launched on 1 APRIL 2024.

  • EJBPS New Impact Factor

    EJBPS Impact Factor has been Increased to 7.482 for Year 2024.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT