Abstract
DETERMINATION OF TENOXICAM IN PRESENCE OF ITS DEGRADATION PRODUCT USING DIFFERENT SPECTROPHOTOMETRIC ANALYTICAL TECHNIQUES

Khalid Abdel-Salam Attia, Mohammed W.I. Nassar and Ahmed H. Abdel-monem*

ABSTRACT

Accurate, selective and sensitive spectrophotometric methods have been developed and validated for determination of tenoxicam in presence of its alkaline degradation product without preliminary separation. These methods include area under the curve method (AUC), Q-Analysis method (QA), Absorbance Ratio method, bivariate method, Ratio difference method and Mean centering method. for the first method (AUC), the area under the curve of mixture solutions in the wavelength ranges 260–275 nm and 275–295 nm were selected for determination of tenoxicam in presence of its degradation product in the range of 2 – 20 μg ml-1 with LOD of 0.282 μg ml-1 and LOQ of 0.854 μg ml-1. for the second method Q-analysis method, two wavelengths 251 nm and 257.4 nm. were selected for determination of tenoxicam in presence of its degradation product in the range of 2 – 20 μg ml-1 with LOD of 0.456 μg ml-1 and LOQ of 1.383 μg ml-1. for the third method Absorbance Ratio method, two wavelengths 251 nm and 364.6 nm. were selected for determination of tenoxicam in presence of its degradation product in the range of 2 - 20 μg ml-1 with LOD of 0.206 μg ml-1 and LOQ of 0.627μg ml-1. for the fourth method bivariate spectrophotometric method two wavelengths 240 nm and 260 nm. were selected by Kaiser method for determination of tenoxicam in presence of its degradation product in the range of 2 – 20 μg ml-1 with LOD of 0.213μg ml-1 and LOQ of 0.711 μg ml-1. for the fifth method ratio difference method is based on measuring the difference in the amplitude of intact tenoxicam in presence of its degradation product at two different wavelengths ,this is done at 250 and 290 nm in the range of 2 – 20 μg ml-1 with LOD of 0.669 μg ml-1 and LOQ of 2.029 μg ml-1. for the sixth method mean centering method; the method was applied for analysis of tenoxicam in presence of its degradation product this is done at 293 nm in the range of 2 – 20 μg ml-1 with LOD of 0.320 μg ml-1 and LOQ of 0.970μg ml-1 The suggested methods were validated as per ICH guidelines and the results revealed that they are reliable, reproducible and precise for routine use with short analysis time. The results obtained by the proposed methods were statistically compared to the reported method, and there was no significant difference between them regarding both accuracy and precision.

Keywords: Tenoxicam, Stability, Area under curve, Q analysis, absorbance ratio, Bivariate method, Ratio difference and mean centering.


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