European Journal of Biomedical and Pharmaceutical Sciences

A COMPARATIVE STUDY OF DIFFERENT APPROACHES FOR STABILITY-INDICATING DETERMINATION OF FLUNIXIN MEGLUMINE IN PRESENCE OF ITS ALKALINE-INDUCED PRODUCT

Khalid Abdel-Salam M. Attia, Nasr Mohammed El-Abasawy and Ahmed Ali Almrasy*

ABSTRACT

Four Simple, rapid, sensitive, accurate and precise stability-indicating spectrophotometric methods were developed for the determination of flunixin meglumine (flunixin-M) in pure and dosage forms. The first method is the second derivative technique; is based on measuring derivatized spectra at 226.8 nm in the range of 5 –50 μg ml-1 with LOD of 0.763 μg ml-1 and LOQ of 2.312 μg ml-1. The second method is the derivative ratio method (1DD); is used for the determination of flunixin-M in presence of its degradation product at 272 nm in the
range of 5 –50 μg ml-1 with LOD of 0.672 μg ml-1 and LOQ of 2.038 μg ml-1. The third method is the ratio difference method; is based on measuring the difference in the amplitude of flunixin-M in presence of its degradation product at two different wavelengths ,this is done at 270 nm and 287 nm in the range of 5 – 50 μg ml-1 with LOD of 0.273 μg ml-1 and LOQ of 0.828 μg ml-1. The fourth method is the mean centering method; the method was applied for analysis of flunixin-M in presence of its degradation product at 288 nm in the range of 5 – 50 μg ml-1 with LOD of 0.260 μg ml-1 and LOQ of 0.790 μg ml-1. The obtained results were statistically compared with those of the reported method by applying t-test and F-test at 95% confidence level and no significant difference was observed regarding accuracy and precision.

Keywords: Flunixin-M, second derivative, derivative ratio, ratio difference, mean centering, pure form, dosage forms, stability-indicating methods