CONTROLLED RELEASE OF GENTAMICIN FROM POLY LACTIC ACID BASED ELECTROSPUN NANOFIBROUS SCAFFOLDS: DESIGN AND OPTIMIZATION
Ardeshirzadeh Behnaz, Noraini Bet Jaafar, Akbari Javar Hamid*, Khosravian Pegah
ABSTRACT
In the present work, Gentamicin loaded- poly (lactic acid) (PLA)/polyethylene glycol (PEG) /poly (N-vinyl-2-pyrrolidone) (PVP) nanofibrous scaffolds were prepared via electrospinning process. The morphology of fibers was characterized by scanning electron microscope. The results indicated that the incorporation of drug into the nanofibers did not show appreciable difference in morphology and average diameter compared with sample without drug. A response
surface methodology based on Box-Behnken design was used to evaluate the effect of key parameters including polymer solution concentration (8-12%), PEG/PLA ratio (10-30%), PVP/PLA ratio and outer thickness of scaffolds (2-10 μm) on the release of Gentamicin from electrospun nanofibrous scaffolds after 48 h and 30 days dissolution tests. Based on results, the entrapment efficiency of Gentamicin from drug-loaded nanofibers was found to be high in all of prepared nanofibrous samples (around to 100%). Furthermore, the release behavior of Gentamicin from nanofibrous scaffolds was depended on the studied parameters. Based on results, the kinetic equation of was well fitted with experimental data. Hence, this study provides a promising Gentamicin loaded PLA/PEG/PVP nanofibers as sustained delivery system for ocular drug delivery in various conditions.
Keywords: Gentamicin, Controlled release, Electrospun nanofibers, PLA/PEG/PVP, Box-Behnken design.
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