DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF NETUPITANT AND PALONOSETRON IN PHARMACEUTICAL DOSAGE FORM
Eali Tejakshi, Yandamuri Narayudu*, Tummapala Devi Prasanna, Valluri Aparna Kumari and P. Abhinava Sri
ABSTRACT
High performance liquid chromatography is at present one of the most sophisticated tool of the analysis. The estimation of Netupitant (NET) and Palonosetron (PAL) was done by RP-HPLC. The separation of NET and PAL was carried on an YMC Pack pro (150 mm x 4.6 mm, 5 μm particle size) c18 column using potassium dihydrogen phosphate: Methanol (70:30 v/v) as the mobile phase with PH (3.0) UV detection was carried out using wavelength 210 nm. The solutions were chromatographed at a constant flow rate of 1.0 ml/min. The linearity range of NET and PAL were found to be from 300-1500 μg/ml of NET and 0.5-2.5 μg/ml of PAL. Linear regression coefficient was not more than 0.999. The values of % RSD are less than 2% indicating accuracy and precision of the method. The total percentage recovery varies from 100.43% and 100.50% for NET and PAL. LOD and LOQ are found to be within the limit. The results obtained on the validation parameters met ICH and USP requirements. It inferred the method found to be simple, accurate, precise and linear. The method was found to be having suitable application in routine laboratory analysis with high degree of accuracy and precision.
Keywords: YMC Pack pro (150 mm x 4.6 mm, 5 ?m particle size) C18 column, NET, PAL, RP-HPLC.
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