ISOLATION AND CHARACTERIZATION OF GLYCYRRHETINIC ACID FROM ROOT OF GLYCYRRHIZA GLABRA
Kaushelendra Mishra* and Girendra Kumar Gautam
ABSTRACT
Glycyrrhiza glabra also well-known as Licorice. It is a famous medicinal herb that grows in various parts of the world and is one of the oldest and broadly used herbs known both in western and eastern countries since several thousand years ago. The triterpenoid saponin, glycyrrhizic acid (glycyrrhizin) was the major bio-active constituent isolated from the roots of Glycyrrhiza glabra (Fabaceae), which is a sweet-tasting material and is about 50 times sweeter than sugar, making it a widely used as a sweetening additive in the food industry. The structure of glycyrrhizin has been characterized as glycyrrhetinic acid. The present study was carried out to isolation and characterization of glycyrrhetinic acid from root of G.glabra from aqueous extract. The chemical compound isolated was analyzed by TLC, HPLC, UV & IR. Glycyrrhetinic acid showed the absorbance maxima at 204nm. The HPLC analysis of isolated sample revealed RT = 2.58 min which is resemblance with standard. Further quantification of glycyrrhetinic acid in the aqueous root extract was also determined by UV & HPLC method.
Keywords: Glycyrrhiza glabra, Glycyrrhetinic acid (GA), TLC, HPLC, UV & IR.
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