European Journal of Biomedical and Pharmaceutical Sciences

CULTIVATION AND CONSTITUENT SCREENING OF MILK MUSHROOM (CALOCYBE INDICA) AND ITS CYTOTOXICITY POTENTIAL AGAINST MCF 7 CELL LINES

Siva R. ? and Kowsalya G.

ABSTRACT

Calocybe indica, commonly known as milk mushroom, is a species of edible mushroom native to India. Mushrooms accumulate a variety of compounds, including carbohydrate, glycosides, phytosterols, phenol, tannins, flavanoids, terpenoids and saponins. Antioxidant compounds prevent oxidative damage related to aging and diseases, such as atherosclerosis, diabetes, cancer and cirrhosis. In this study, Calocybe indica was cultivated on three different substrates namely paddy straw, sugarcane bagasse and cocopeat: rice bran (1:1). Chemical constituents present in Calocybe indica were analysed and cytotoxicity were screened against cancer cell lines. The results showed that the cocopeat: rice bran (1:1) substrate resulted higher yield with high amount of Carbohydrate, Protein and Vitamin A than paddy straw and sugarcane bagasse as a substrate. Constituent analysis showed higher concentration of phenols, flavonoids, tannins, terpenoids, saponins and absence of glycosides and alkaloids. Different concentrations of Calocybe indica (1ng, 10ng, 100ng, 1g, 10g, 100g) were evaluated against MCF 7 cell lines for cytotoxicity studies by MTT assay. In all cases crude extract of 100g showed maximum cell apoptotic potential than the rest.

Keywords: Cytotoxicity, Calocybe indica, MTT assay, coco peat, cancer cell lines.