European Journal of Biomedical and Pharmaceutical Sciences

EVALUATION OF ANTIDIABETIC ACTIVITY OF CUCURBITA PEPO, CORALLOCARPUS EPIGAEUS AND THEIR FORMULATION IN EXPERIMENTAL RATS.

*Javeed Ahmad Tantray, Syed Basheer uddin, Dr. K. Pratap Reddy, Kaiser Jamil.

ABSTRACT

Diabetes has become a common global health problem that affects >170 million people worldwide. It is one of the leading causes of death and disability. T2D has become an epidemic in the 21st century where India leads the world with largest number of diabetic subjects. The present study was planned to evaluate the antidiabetic activity of cucurbita pepo, Corallocarpus epigaeus and their formulation in streptozotocin induced diabetic experimental rats. From this the new information about the plant possessing antibiotic activity may be delivered which may be used for the future studies in the drug discovery and development. Wistar albino rats of either sex, 8 to 10 weeks old, weighing about 180-200gm were used in experiments. Filtrate extract of cucurbita pepo and corallocarpus epigaeus was used for various phytochemical screening. The acute toxicity for aqueous extract of fruit of cucurbita pepo, ethanolic extract of tuberous root of corallocarpus epigaeus and their formulation were determined. The rats was made diabetic by an intra-peritoneal (i.p) injection of streptozotocin at a dose 60mg/kg b.w. Assessment of blood glucose levels, total cholesterol, total triglyceride, HDL, LDL,VLDL,SGPT,SGOT, Serum urea, serum creatinine levels were done. In glucose tolerance test, the glucose levels were estimated before drug treatment and at different intervals thereafter. Administration of extracts in separate manner decreased the blood glucose level to near normal control but treatment with composite extract formulation showed better decrease in blood glucose level. In case of serum lipid profile, it was observed that there was an increase in serum total cholesterol, serum total glyceride, LDL, VLDL, SGPT, SGOT, Serum urea and serum creatinine whereas a decrese in HDL levels in diabetic rats. After 21 days continuous administration of aqueous extract of fruit of cucurbita pepo (200mg/kg b.w p.o), ethanolic extract of tuberous root of corallocarpus epigaeus (50mg/kg b.w p.o), their formulation (100mg/kg b.w p.o) in diabetic rats led to significant decrease in serum total cholesterol, serum total glyceride, LDL, VLDL, SGPT, SGOT, Serum urea and serum creatinine levels in diabetic rats, while HDL levels were increased. Recovery was better in the composite extract than the individual extract treated group. The present study indicates that the aqueous fruit extract of cucurbita pepo, ethanolic extract tuberous root of corallocarpus epigaeus and their formulation possess the anti diabetic activity. Formulation was found to be more effective than aqueous fruit of Cucurbita pepo and ethanolic extract of tuberous root of corallocarpus epigaeus administered alone.

Keywords: Diabetes mellitus, Corallocarpus epigaeus, cucurbita pepo, streptozotocin, extracts, blood glucose.