European Journal of Biomedical and Pharmaceutical Sciences

ADIPOKINES (ENDOTHELIAL LECTIN HL-1 "OMENTIN-1") CHANGES AND PCOS PATIENTS SEVERITY AND CLASSIFICATIONS

Dr. Ali Hassan Mohammed*

ABSTRACT

Subject: If Adipokines a good measurments can predict severity of PCOS patients. Methods Samples: After fasting overnight, the venous blood samples were aspirated at 08:00-10:00 am during the 3rd-6th day of the menstrual cycle (early follicular phase) for patients with normal cycles. For patients with ovulation or oligomenorrhea, blood samples were collected regardless of the duration of the cycle. The samples were transferred into clean, plain tubes and centrifuged within 30 minutes of collection. Then the serum from all blood samples were separated; sugar and lipid profile levels were measured using enzyme colorimetric methods and the rest were stored at 18°C until the time of assay. Patients: 60 infertile Iraqi women aged 18-37 years (M±SD: 26.45±4.65) were enrolled for the study. Their medical histories were noted, body weight and height were measured, and body mass index (BMI) was calculated [mean BMI (34.01±3.54 Kg/m2)]. PCOS patients had already been diagnosed and the diagnosis was confirmed according to the European society of human reproduction and embryology and American society for reproductive medicine criteria; PCOS is diagnosed if there are any two of the following. • Presence of polycystic ovary on ultrasound examination. • Clinical or biochemical hyperandrogenemia. • Menstrual dysfunction with an ovulation. Healthy Control Group: For comparison, thirty apparently healthy fertile women with regular cycles who matched for age, weight, and BMI [n=30; age=28.87± 3.27 (years); BMI=29.56 ± 2.12 (kg/m2); mean ± SD] were also included as control subjects. Protocol: Clinical and anthropometrical variables, including clinical blood pressure, BMI, (calculated as kg/m2) and waist-to-hip ratio (WHR) were determined in all the subjects. Omentin-1, IL6, IL-18, resistin, TNF-a, ghrelin, RBP-4 leptin, insulin, LH, FSH, total testosterone, free testosterone and sex hormone-binding globulin (SHBG) were determined by ELISA methods. Serum fasting glucose level was measured by enzymatic method supplied by Giesse Diagnostics. The homeostasis model assessment (HOMA) was used to calculate an index of insulin resistance for each patient, and beta-cell function was estimated from fasting insulin and glucose levels by the homeostasis model assessment (HOMA-b). Using the fasting glucose (mmol/L) and insulin (μIU/ ml), the index for insulin resistance; HOMA IR, was defined as (insulin ×glucose)/22.5 and HOMA b-cell function × 20 × insulin/(glucose-3.5)(22). Statistics: All statistical analysis in the study were performed using SPSS version 15.0 for Windows (Statistical Package for Social Science, Inc., Chicago, IL, USA). Descriptive analysis was used to show the mean and standard deviation of the variables. The significant difference between mean values was estimated by the Student t-test. The point of statistical significance was noted when probability was p<0.05, and no statistical significance was noted when p>0.05. Correlation analysis was used to test the linear relationship between parameters. ANOVA test was used to show the differences between variables of differentiated groups. Results: Compared with normal controls, the results showed that women with PCOS had increased (p<0.001) levels of IL-6, IL-18, TNF-a, RBP-4, Resistin, leptin, insulin, LH, testosterone, free testosterone, HOMA, and HOMA ß-cell% (Table 1) Furthermore, patients with PCOS also had lower omentin-1 (p<0.001), ghrelin(p<0.05) and QUICKI (p<0.001) values than controls (Table 1), while FSH and FBG levels were not significantly different (p>0.05) between the two groups. Conclusion: Omentin levels may be predictive of the metabolic consequences or co-morbidities associated with obesity.

Keywords: IL-6, IL-18, TNF-a, RBP-4, Resistin, leptin, insulin, LH.