European Journal of Biomedical and Pharmaceutical Sciences

COMPARISON OF IN VITRO ANTIOXIDANT ACTIVITY OF CORIANDRUM SATIVUM LINN FRUIT BY SOXHLATION AND MICROWAVE EXTRACTION TECHNIQUE

Padmaa M Paarakh*

ABSTRACT

The aim of the present study is to compare the in vitro antioxidant activity of Coriandrum sativum fruit by two different technique of extraction viz., Soxhlation and Microwave extraction and correlate the antioxidant activity with the amount of flavonoids and phenol content present in the extract. Coriandrum sativum fruits were extracted separately in soxhlet and microwave extractor with distilled water, 25% ethanol, 50% ethanol, 75% ethanol and ethanol respectively. The study were carried out with all 5 extracts by both methods using different in vitro antioxidant model viz., Phosphomolybdenum antioxidant assay, Reducing power assay and DPPH radical scavenging assay. Total flavonoid content and phenol content were also determined. The study exhibited strong antioxidant activity in different in vitro systems. The 75% ethanol extract showed better results than all other 4 extracts followed by 50% ethanol and 25% ethanol extract on evaluation with the different in vitro antioxidant methods. Flavonoid content and phenol content also correlated well the in vitro antioxidant activity. Microwave extraction values were better when compared to soxhlet technique. This experiment has concluded the strong in vitro antioxidant properties of C. sativum. 75 % ethanol showed the highest antioxidant activity when compared all other four extracts. Microwave extraction technique is better than soxhlet technique of extraction. The total flavonoid content and phenol content were also more when microwave extraction technique was used. Further, investigation on in vivo antioxidant activity has to be carried out to understand its mode of action and to discover the main constituent of C.sativum fruit responsible for this antioxidant effect.

Keywords: Coriandrum sativum, antioxidant activity, soxhlet extraction, microwave extraction, phosphomolybednum antioxidant assay, reducing power assay, DPPH assay.