European Journal of Biomedical and Pharmaceutical Sciences

COPPER SULPHATE INDUCED EXPRESSIONS OF DIGESTIVE ENZYMES AMYLASE, PROTEASE AND LIPASE IN INVERTEBRATE MODEL: BELLAMYA BENGALENSIS (L).

*Kamble N. A.

ABSTRACT

Pollution is one of the undesirable side effects of increased industrialization, generally responsible for aquatic contamination. Heavy metals are major pollutants released into the environment. Biotoxicity of these metals against enzyme reactivity provides information about animal’s health. Present investigation deals with intoxication of pre-determined mean LC50 concentration of heavy metal Copper sulphate (CuSO4. 5H2O) as 0.56 ppm, for 24 hrs., 48 hrs., 72 hrs. and 96 hrs. Three different enzymes as amylase, lipases and protease activity were biochemically assessed from five different digestive organs including glands. Comparative data of enzyme expression against toxicity of heavy metal was interpreted. Enzymatic reactions were found significantly reduced as per exposure period and toxic dose. Results obtained were statistically documented and correlated with change in rate of biochemical reactions in invertebrate aquatic molluscan model as snail Bellamya bengalensis (L).

Keywords: Enzyme expression, digestive organs, Copper sulphate, physiology of digestion, Bellamya bengalensis.