European Journal of Biomedical and Pharmaceutical Sciences

ISOLATION AND CHARACTERIZATION OF CHEMICAL CONSTITUENTS FROM THE LEAVES OF AGERATUM CONYZOIDES L. AND JASMINUM SAMBAC (L.) SOL. AND FRUITS OF PYRUS COMMUNIS L.

Shahnaz Sultana, Mohammed Ali*, Showkat Rassol Mir and Arun Mittal

ABSTRACT

Ageratum conyzoides L. (Asteraceae) is found in Central and South America, Africa, Australia and south-eastern Asia. It is used to cure colds, coughs, dyspepsia, eye diseases, headache, leprosy, malaria, pneumonia, rheumatism, skin, stomach and uterine disorders, sleeping sickness, snake bites and wounds. Jasminum sambac (L.) Sol. (Oleaceae) is distributed in north-eastern India and used to treat dysentery, epilepsy, fever, insanity, galactorrhoea, impotency, ophthalmic, respiratory and skin problems and body aches. Pyrus communis L. (Rosaceae) grows in central and eastern Europe and southwest Asia. Its fruits are taken to relieve arthritis, diarrhoea, cholera, colic, colitis, constipation, dropsy, fever, gallbladder disorders, gout, nausea, sclerosis, spasms and tumours. Our study was planned to isolate chemical constituents from the leaves of A. conyzoides and J. sambac and fruits of P. communis and to characterized their structures. Phytochemical investigation of a methanolic extract of the leaves of A. conyzoides gave (Z)-n- tripentacont-43-ene-22-one (1). The leaf methanolic extract of J. sambac afforded β- D- glucose (2), glyceroloxy glycerol (3) and three new acyl glycosides characterized as lauryl O-β-D- xylopyranoside (4), n-tridecan-7β-olyl O-β-D- arabinopyranosyl-(2ʹ→1″)-O-β-D- arabinopyranoside (n-tridecan-7β-olyl O-β-D-diarabinoside, 5) and oleiyl O-β-D-arabinopyranosyl-(2ʹ→1″)-O-β- D- glucopyranoside (oleiyl O-β-D-arabinoglucoside, 6). A methanolic extract of the fruits of P. communis on subjection to silica gel column furnished gadoleic acid (7), oleiyl-O-β-D-glucopyranosyl-(6a→1b)-O-β-D-glucopyranosyl-(6b→1c)-O-β-D-glucopyranosyl-(6c→1d)-O-β-D-glucopyranoside (oleiyl β-D-tetraglucoside, 8) and oleiyl-O-β-D-glucopyranosyl-(6a→1b)-O-β-D-glucopyranosyl-(6b→1c)-O-β-D-gluco - pyranosyl-(6c→1d)-O-β-D-glucopyranosyl-(6d→1e)-O-β-D-glucopyranosyl-(6e→1f)-O-β-D-glucopyranoside (oleiyl β-D-hexaglucoside, 9). The structures of all the isolated phytoconstituents have been established on the basis of spectral data analysis and chemical reactions.

Keywords: Ageratum conyzoides leaves, Jasminum sambac leaves, Pyrus communis fruits, Chemical constituents, Isolation, Characterization.