OPTIMIZATION OF METSCHNIKOWIA PULCHERRIMA GROWTH IN DISTILLERY SPENT WASH FOR BIODIESEL PRODUCTION
Yasmin Regina M.*
ABSTRACT
Biodiesel is a renewable fuel which synthesis from wastewater is a promising alternative to fossil fuels that are limited in availability. Organic molecules of wastewater contain a lot of chemical energy. Waste to energy is the most preferred technique in which wastewater used as a substrate for some microorganisms that can store lipids within their cells. Microbial oil is a renewable alternative to petroleum and diesel fuels as well as microorganisms utilize waste water instead of food crops or other crops for biodiesel production. Distillery spent wash, high organic load wastewater is used for growing Metschnikowia pulcherrima oleaginous yeast which accumulates lipids of 40% of their dry weight and the lipids converted to non-toxic and biodegradable biodiesel (Fatty Acid Alkyl Esters) by trans-esterification. It is beneficial to environment by reducing the waste and converting it into energy source. In this paper, optimization of growth condition of M. pulcherrima was analyzed for static and shaking condition, different pH values and incubation periods. 0.466 g/L lipid yield obtained from distillery spent wash in the optimum condition of pH-6.0, 25ᴼC temperature and shaking at 160rpm.
Keywords: biodiesel, distillery spent wash, anaerobically, trans-esterification, oleaginous yeast, biodegradable, Fatty Acid Alkyl Esters.
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