CELL CYCLE ARREST AND APOPTOSIS INDUCING POTENTIAL OF INDIAN HONEY IN CERVICAL CANCER CELLS
Naik Amruta*, Dr. Dandekar Sucheta and Dr. Naik Nishigandha
ABSTRACT
Honey is a natural product known for its various biological activities like anti-inflammatory, antioxidant, antibacterial, anticancer effects. Cancer is one of the leading causes of death worldwide. Honey due to the presence of phytochemicals in it is found to inhibit the cancer growth. This effect is mediated trough cell cycle arrest and induction of an apoptosis. This study focused on the effect of four Indian unifloral honey i.e. Jambhul, Rubber, Litchi and drumstick honey on cervical cancer cell line. The cell cycle arrest were analysed by Propidium Iodide (PI) staining using Flow Cytometer. Further confirmation of apoptosis was done by microscopic examination of stained cells. In order to understand the mechanism of this effect, expression of important marker protein of apoptosis i.e. Active Caspase-3 was analyzed by flow cytometry. All honey samples caused cell cycle arrest in G2M phase in cells. Sub G0G1 peak indicating apoptosis was confirmed by microscopic examination in which apoptic bodies were observed. Prominent increase in expression of Active Caspase-3 protein was seen. Hence, this study concludes that all four Indian honey samples showed potent anticancer activity against cervical cancer.
Keywords: Honey, Cell cycle, Apoptosis, Caspase 3.
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