IDENTIFICATION OF THE ANTI-NOCICEPTIVE COMPOUNDS FROM STEM BARK EXTRACT OF ERYTHROPHLEUM IVORENSE USING GAS CHROMATOGRAPHY MASS SPECTROSCOPY (GC-MS) TECHNIQUES
Wakeel O. K.*, Elusiyan C. A., Adewole T. A., Adeyaba O. A. and McDonald A.
ABSTRACT
Previous study revealed the anti-nociceptive activity of bioassay-guided fractionation of semi-purified fraction of E. ivorense stem bark, but the bioactive compounds responsible have not been identified. Hence, this study was designed to unravel the bioactive compound behind the reported pharmacology function using GC-MS techniques. The genus Erythrophleum ivorense belonging to the family fabaceae is a small deciduous, soft wooded tree. The stem bark extract of Erythrophleum ivorense has long been used in different ayurvedic preparations in the treatment of many ailments like small pox, swollen, emetic, convulsion, body pain, and as anthelmintic. A comparative study of compounds presents in the semi-purified fractions obtained from ethyl acetate fraction of Erythrophleum ivorense stem bark extract through gas chromatography mass spectroscopy was performed. In the GC-MS analysis, thirty-four bioactive compounds were found in semi-purified fractions (F3C1, F3C2, F3C3, F3C4, F3C5-crystal and F3C5-MQ). Twelve were isolated from the F3C5-MQ semi-purified fraction, while F3C1 has the least (2) compounds. Palmitic acid (C17H34O2), Linoleic acid (C19H34O2), Oleic acid (C19H36O2) were very common in F3C2, F3C3, and F3C5-MQ respectively, also, Erythroformide (C30H46O) were found in F3C2, F3C3, and F3C4. α-amyrin (C30H50O), Stearic acid (C19H38O2), Behenic acid (C23H46O2), Lignoceric acid (C25H50O2) and 9-Hexadecenoic acid (C17H32O2), were found in F3C5-MQ only, While, non was found in the fraction F3C5-crystal. This study showed that among the five semi-purified fractions of stem bark extract of Erythrophleum ivorense, the highest amount of bioactive compounds found in F3C5-MQ might be responsible for the central and peripheral anti-nociceptive effect of the fraction previously reported. Further advance study is required for structural analysis and accurate quantification of compounds in the stem bark extract of Erythrophleum ivorense.
Keywords: Bioactive compounds, stem bark extract, isolation, Erythrophleum ivorense, GC/MS.
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