European Journal of Biomedical and Pharmaceutical Sciences

CHLORAMBUCIL -TREATMENT INDUCED OXIDATIVE STRESS IN TESTICULAR TISSUE OF SWISS ALBINO MICE

Olajumoke Omolara Ojo*

ABSTRACT

Chlorambucil (CLB) is an alkylating agent used against chronic leukemia. This study was carried out to investigate the adverse effect of chlorambucil treatment on testicular tissue of mice. Twenty Swiss albino mice were randomized divided into four groups (n=5). Group 1 served as control received vehicle while 2, 3 and 4 groups received 1mg/kg, 3mg/kg and 5mg/kg body weight of chlorambucil (CLB) respectively for 28 days via intraperitoneal injection. Testosterone and Luteinizing hormone were estimated. Oxidative and anti-oxidative biomarker enzymes such as lipid peroxidation (LPO), catalase (CAT), superoxide dismutase (SOD) and reduced glutathione (GSH) levels were evaluated. Effect on sperm parameters was carried out. The results showed that treatment with chlorambucil at 3mg/kg and 5mg/kg caused a significant increase at p>0.05 in the luteinizing hormone and testosterone levels. CLB at 3mg/kg and 5mg/kg body weight caused significant increase in LPO with simultaneous decrease in CAT, SOD and GSH levels. The findings revealed that chlorambucil treatment resulted in generation of oxidative stress in the testicular tissue of treated mice in dose dependent manner.

Keywords: Chlorambucil, Oxidative stress Testis, Mice, Reproductive toxicity.