Abstract
DESIGN PRIMER GENE TET(M) LACTOBACILLUS PLANTARUM

Rafika Sari*, Fathu Rahman and Robiyanto

ABSTRACT

Tetracycline is an antibiotic that reported have resistance to some infectious bacteria. One of the mechanisms of tetracycline antibiotic resistance is the ribosomal protection through the tet(M) gene. The tet(M) gene has also been identified in the probiotic bacteria Lactobacillus plantarum. Nucleotide tet(M) is used to design primers to be an important element in the technique of DNA amplification in the Polymerase Chain Reaction (PCR) method, it can be a useful diagnostic before using tetracycline antibiotics in therapy. The method used for designing primer tet(M) gene from Lactobacillus plantarum is by using nucleotide sequence data obtained from NCBI, which then obtained primer candidates using Primer3plus, and primer candidates analyzed of primer criteria contained on it with OlygoAnalyzer 3.1. The results obtained, there are five primer candidates and produce one best primer is primer number 4 with primer forward - GAC ACG CCA GGA CAT ATG GA - and primer reverse - GAC GGA CCT CGA TGT GTT GA -.

Keywords: Tetracycline, tet(M), Lactobacillus plantarum, Primer.


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