Abstract
RAPID DETECTION OF DRUG RESISTANT TUBERCULOSIS BY DIRECT NITRATE REDUCTASE ASSAY IN RESOURCE POOR SETTINGS

Dristi Halwai*, Rajendra Gurung and S. K. Bhattacharya

ABSTRACT

Background: The most common method for detection of drug resistant tuberculosis is conventional proportion method requiring three months to yield the results. Delay in the proper diagnosis may hinder the effective treatment and increase the transmission of the drug resistant tuberculosis. Hence, this study was conducted to evaluate nitrate reductase assay (NRA) as a simple and rapid alternative method for detecting drug resistance tuberculosis in resource limited settings. Methods: A total of fifty eight new smear positive sputum samples were processed as per the standard guidelines. After decontamination, sputum samples were inoculated on NRA medium for rapid drug susceptibility testing and conventional culture medium. Results: NRA detected 13.2% isoniazid resistance, 11.3% rifampicin resistance and 5.7% multidrug resistant strains of Mycobacterium tuberculosis. A total of 44 samples yielded results within 21 days and 19 samples yielded results in 14 days. The mean duration for detection and drug susceptibility testing of Mycobacterium tuberculosis by NRA was 19.2 days. Conclusions: The present study concludes that NRA is a simple and rapid alternative method for detection of drug resistance to isoniazid and rifampicin in resource strain settings.

Keywords: Drug resistant tuberculosis, nitrate reductase assay, tuberculosis.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tanima Debnath Sarkar

Download Article : Click here

News & Updation

  • EJBPS: OCTOBER ISSUE PUBLISHED

    OCTOBER 2021 Issue has been successfully launched on 1 October 2021

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 5.467 to 6.044, due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT