EVALUATION OF SUBACUTE ORAL TOXICITY INDUCED BY ETHANOLIC EXTRACT OF DALBERGIA SISSOO LEAVES IN EXPERIMENTAL RATS
Mrunal M. Vaidya*, Swati U. Kolhe and Sachin V. Tembhurne
ABSTRACT
Herbal medicine is the source for the search of many novel therapeutic compounds in developing countries. Before used as medicine, drugs from plant origin must be ensured safe. The study is aimed at evaluating the possible toxicity in 28-day subacute oral toxicity of ethanolic extract Dalbergia sissoo (D. sissoo) in male and female Wistar rats. The 28-day subacute toxicity study was conducted to detect the no-observed adverse effect level (NOAEL). In this study, a total of 48 rats were divided into the control, low dose (200 mg/kg), medium dose (500 mg/kg) and high dose (1000 mg/kg) groups. The extract was administered daily from day 1 until day 28. At the end of the study, the animals were humanely sacrificed and assessed for the effect extract of Dalbergia sissoo leaves on body weight and relative organ weights and hematological, biochemical and histopathological parameters. The hematological and serum biochemical parameters for the assessment of kidney and liver injuries were carried out. Results of hematological and serum biochemistry results showed no changes in the control and treated groups. In the histopathology, evaluation of kidney tissues in all treated groups showed no significant (p > 0.05) lesions.
Keywords: Subacute oral toxicity, Dalbergia sissoo, biochemical analysis, hematological parameters, histopathology.
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