European Journal of Biomedical and Pharmaceutical Sciences

STABILITY STUDY OF MADHUMEHARI VATI USED IN THE MANAGEMENT OF TYPE 2 DIABETES (MADHUMEHA) - WITH RESPECT TO BASELINE MICROBIAL DIAGNOSTIC MODALITIES

Sachin Kumar Sharma*, Alankruta R. Dave, Brijbala Vasishth, M. S. Cholera, Vasundhara Sharma.

ABSTRACT

Background: Diabetes mellitus (DM), is a group of metabolic disorders characterized by high blood sugar levels over a prolonged period. In Ayurveda disease Diabetes Mellitus can be correlated with Madhumeha. From different drugs described in Ayurveda for the management of Madhumeha (Type 2 Diabetes), a compound formulation has been made named Madhumehari Vati. In this formulation whole plant extract of Mamajjak (Enicostemma littorale), leaves extract of Meshashringi (Gymnema sylvestre), nut extract of Latakaranja (Caesalpinia bonducella), root extract of Katuki (Picrorhiza kurroa), fruit extract of Pippali (Piper longum), fruit extract of Rakta-maricha (Capsicum frutescens), fruit extract of Indravaruni (Citrullus colocynthis) were taken. Aim: To study the stability of Madhumehari Vati with respect to its microbial profile. Materials and Methods: Prepared sample of Madhumehari Vati was studied to check microbial contamination at regular time intervals. Tablets were studied at regular intervals of 1 month for a period of 09 months to analysis Mycological findings and presence of bacteriological findings by Wet mount preparation and Gram stain test respectively. Results: Sample was subjected to the microbiological study from the date of the preparation to the date of last microbiological study. No any contaminations were found in microbiological study. Discussion: Hence the present Study was carried out to observe the stability study of Madhumehari Vati with respect to Microbial Contamination of sample prepared and store in different climatic conditions and temperature. Thus a baseline Microbial profile was studied at regular interval of 1 month for total 11 month (i.e. time for consumption of prepared drug). At the end of study it was found that sample was not showed presence of any Microbes. Conclusion: In microbiological study, Tablets container has not present of microbes after 11 months of preparation sample, even in different climate and temperature, shows its stability and good shelf life. Hence in present study the stability test of Madhumehari Vati with respect to microbiological findings was negative at room temperature, warm and cold, dry and humid conditions.

Keywords: Ayurveda, Climate conditions, Microbial profile, Stability, Type 2 Diabetes (Madhumeha), Madhumehari Vati.