EFFECT OF EXTRACTION SOLVENTS ON IN VITRO ALPHA AMYLASE INHIBITORY ACTIVITY OF COSTUS SPECIOSUS LEAVES
Tanaya Ghosh, Prasenjit Mitra and Prasanta Kumar Mitra*
ABSTRACT
Alpha amylase inhibitory activity of Costus speciosus (C. speciosus) leaves is known in literature. But conflicting reports are available on alpha amylase inhibitory activity of different solvent extracts of C. speciosus leaves. Aim of the present work was to see the effect of extraction solvents on in vitro alpha amylase inhibitory activity of C. speciosus leaves. Leaves of C. speciosus were collected from the local market and identified by the taxonomist. Solvent extractions of the leaves were made separately by using chloroform, petroleum ether, ethanol, isopropanol and hexane. Extracts were separately dried and processed for in vitro alpha amylase inhibitory activity by standard method. Acarbose, an alpha amylase inhibitor, was used as control. Results showed that ethanol extract of C. speciosus leaves had maximum alpha amylase inhibitory activity in comparison to that of other solvent extracts. In vitro alpha amylase inhibitory activity of ethanol extract of C. speciosus leaves (IC50 value 43.3±1.0 μg/mL) was comparable to that of acarbose (IC50 value 47.2±1.0 μg/mL). This study therefore suggests uses of ethanol extract of C. speciosus leaves in the management of diabetes.
Keywords: Costus speciosus leaves; extraction solvent extractions; alpha amylase inhibitory activity, acarbose.
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