European Journal of Biomedical and Pharmaceutical Sciences

DNA BINDING AND ANTIBACTERIAL ACTIVITY OF BIVALENT METAL COMPLEXES WITH 1,10- PHENANTHROLINE AND 2- ACETYLTHIOPHENE THIOSEMICARBAZONE

Kummara Srinivasulu, D. Dhanalakshmi, K. Anuja and Katreddi Hussain Reddy*

ABSTRACT

Bivalent metal complexes having the composition M(Phen)2Cl2 (where, M= Cu(II), Ni(II) and Co(II); Phen = 1,10-Phenonthroline) are reacted with 2-acetylthiophene thiosemicarbazone (ATT) to produce bivalent metal complexes with molecular formula [M(Phen)2ATT]Cl. The complexes are characterized using physical (molar conductivity) and spectral (mass spectra, infrared and electronic spctroscopies) methods. Electrochemical behaviour of complexes was uncovered using cyclic voltammetry. The complexes show quasi reversible cyclic voltammetric responses for the Cu(II)/Cu(I) couple. The DNA binding properties of the complexes are determined by using UV-Visible absorption spectrophotometry. Metal complexes are screened for their antibacterial activity by using agar well diffusion method against pathogenic bacterial strains viz. Gram –ve such as Escherichia coli, Klebsiella pneumonia and Gram +ve such as Staphylococcus aureus, Bacillus cereus. Anti bacterial activity of the present complexes are comparable with the activity of Ciprofloxacin. The [Cu(Phen)2(ATT)]Cl 1.5 H2O complex inhibits bacteria more strongly than any other complex. The [Co(Phen)2(ATT)]Cl complex shows more activity than the parent complex, Co(Phen)2Cl2.

Keywords: Bivalent metal complexes, 2-Acetylthiophene thiosemicarbazone, DNA binding, Antibacterial activity.