Abstract
ASSESSMENT OF ANTIOXIDANTS AND ANTIMICROBIAL EFFICACY OF BIOACTIVE METABOLITES ISOLATED FROM PSEUDOMONAS MONTEILII

Thampy Aditya Sreekumaran and S. Umamaheswari*

ABSTRACT

The aim of the work deals with assessment of the antioxidants and antimicrobial activity of crude bacterial culture Pseudomonas monteilii collected from the Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu. Hydroxyl radical scavenging assay is based on the qualification of the degradation product of 2 deoxy ribose by condensation with TBA. The radical scavenging activity of different extracts was determined by using DPPH assay. The decrease in the absorption of the DPPH solution after the addition of the sample was measured at 517 nm. Ascorbic acid (10mg/ml DMSO) was used as reference. 1, 1-diphenyl-2-picryl hydrazyl is a stable free radical with pink color which turns yellow when scavenged. Antioxidants react with DPPH and reduce it to DPPH-H and as a consequence the absorbance decreases indicating the scavenging potential of the antioxidant compounds in terms of hydrogen donating ability. The antibacterial analysis of the crude extract of Pseudomonas monteilii was evaluated and was found to exhibit activity against both Gram-positive (Bacillus cereus) and Gram-negative (Escherichia coli) bacteria while the antifungal efficiency of the crude sample against the isolated fungus from the homemade pickle was investigated with their minimum inhibitory concentration. Hence the bioactive metabolites isolated from Pseudomonas monteilii proved to be potentially effective and can be used as biopreservatives such as bacteriocin that can preserve food stuffs avoiding health hazards of chemically antimicrobial agent applications.

Keywords: Bioactive Metabolites, Pseudomonas monteilii, Hydroxyl Radical, DPPH, Biopreservatives, Bacteriocin.


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