ELECTROPHEROTYPES OF ROTAVIRUS ISOLATED FROM CHILDREN IN KHARTOUM TEACHING HOSPITAL
Salma Ahmed Mukhtar Egool*, Dr. Isam M. Elkhider, Prof. Muntaser E. Ibrahim
ABSTRACT
The aim of this study was to reveal percentage of rotavirus infection
among infants and young children in Khartoum Teaching Hospital.
Material and method: an observation descriptive study was designed
and a total of 150 children of age less than 5 years, who had acute
diarrhea were studied from 2002 to 2004 at Khartoum Teaching
Hospital. Result: rotavirus antigens were detected in 24.6 % (37of
150) of fecal specimens collected from children when tested by
Enzyme Linked Immuno sorbent Assay. Twenty five specimens of
ELISA positive were positive by polyacrylamide gel electrophoresis (RNA -PAGE).
Polyacrylamide gel electrophoresis (RNA -PAGE) test; all samples showed long
electropherotypes patterns except one that showed short electropherotype pattern. This is the
first report on common electropherotypes of rotavirus infection among children in Sudan.
This implies that RNA-PAGE is a more specific and reliable method than ELISA for
detecting rotavirus in fecal samples. Further work is needed for a better understanding of
rotavirus molecular epidimiology and its vaccination.
Keywords: Rotavirus,electropherotypes.khartoum, children.
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