Abstract
ELECTROPHEROTYPES OF ROTAVIRUS ISOLATED FROM CHILDREN IN KHARTOUM TEACHING HOSPITAL

Salma Ahmed Mukhtar Egool*, Dr. Isam M. Elkhider, Prof. Muntaser E. Ibrahim

ABSTRACT

The aim of this study was to reveal percentage of rotavirus infection among infants and young children in Khartoum Teaching Hospital. Material and method: an observation descriptive study was designed and a total of 150 children of age less than 5 years, who had acute diarrhea were studied from 2002 to 2004 at Khartoum Teaching Hospital. Result: rotavirus antigens were detected in 24.6 % (37of 150) of fecal specimens collected from children when tested by Enzyme Linked Immuno sorbent Assay. Twenty five specimens of ELISA positive were positive by polyacrylamide gel electrophoresis (RNA -PAGE). Polyacrylamide gel electrophoresis (RNA -PAGE) test; all samples showed long electropherotypes patterns except one that showed short electropherotype pattern. This is the first report on common electropherotypes of rotavirus infection among children in Sudan. This implies that RNA-PAGE is a more specific and reliable method than ELISA for detecting rotavirus in fecal samples. Further work is needed for a better understanding of rotavirus molecular epidimiology and its vaccination.

Keywords: Rotavirus,electropherotypes.khartoum, children.


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