European Journal of Biomedical and Pharmaceutical Sciences

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC FOR ESTIMATION OF IVABRADINE AND METOPROLOL IN PURE AND PHARMACEUTICAL DOSAGE FORM

K. Sai Durga*, L. Ramachandra Reddy, D. Dhachinamoorthi

ABSTRACT

A simple, Accurate, precise method was developed for the simultaneous estimation of the Ivabradine and Metoprolol in tablet dosage form. Chromatogram was run through Discovery C8 250mm x 4.6 mm, 5m. Mobile phase containing KH2PO4: Acetonitrile taken in the ratio 55:45 was pumped through column at a flow rate of 1.0 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 260.0nm. Retention time of Ivabradine and Metoprolol were found to be 2.517min and 3.097min. %RSD of the Ivabradine and Metoprolol were and found to be 0.6 and 0.3 respectively. %Recovery was obtained as 99.65% and 99.39 % for Ivabradine and Metoprolol respectively. LOD, LOQ values obtained from regression equations of Ivabradine and Metoprolol were 0.31, 0.95 and 0.68, 2.06 respectively. Regression equation of Ivabradine is y = 17301x + 1713 and y = 17366x + 3213 of Metoprolol. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

Keywords: Ivabradine, Metoprolol, RP-HPLC.