THE POSSIBLE PROTECTIVE ROLE OF NIGELLA SATIVA METHONELIC EXTRACTION AGAINST IONIZING RADIATION INDUCED CERTAIN HEMATOLOGICAL STRUCTURAL AND CONFORMATIONAL ALTERATION IN RATS
Shaban Alramlawy*, M. Ali, Khedr M. H., Helal N., R.A.M. Rizk
ABSTRACT
The present work aimed to investigate the protective effect of Nigella sativa methonelic extraction against gamma radiation effect on the hematological parameters in male rats. Method: Sixty Male rats divided into 12 groups, 5 each, were used in this work. Average weighing 200-250 g were used. The rats were kept in in special designed cages and maintained under constant air flow and illumination during the experimental periods. Group 1 served as vehicle control, Group 2 served as NS group. Five Groups were injected intravenously with Tc-99m, while other five groups were orally administrated with Nigella sativa (400 mg/kg) then injected intravenously with Tc-99m.All these groups were decapitated after 7, 14, 30 and 60 days respectively. Some hematological parameters were determined for all groups, including red blood cells (RBCs), white blood cells (WBCs), platelets (PLT), Hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC). RESULTS: The findings results showed a disturbance in the levels of the estimated hematological parameters for all groups (P< 0.05) compared to the control group. The results of UV spectrum show partial photo dissociation. showed a decrease in intensity, all irradiated serum sample intensity was less than control sample; this result indicates that there is photo degradation happened to the blood components, this causes changes in the structure and conformational changes. Also Results revealed that administration of Nigella sativa methonelic extraction has protective effects against radiation-induced damage. CONCLUTION: The present study clarified that oral administration of Nigella sativa methonelic extraction is beneficial to prevent alterations induced by gamma radiation.
Keywords: Gamma radiation, hematological parameters, Nigella sativa, UV-spectroscopy.
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