European Journal of Biomedical and Pharmaceutical Sciences

SEQUENCE ANALYSIS OF 16S RDNA GENE IN POULTRY AEROMONAS ISOLATES

Disouky M. Mourad* and Hanan S. El-Samahy

ABSTRACT

Aeromonas species have more than one genomovar (DNA group) which cannot be separated phenotypically, so that this study was conducted to determine and differentiate genomic variation, phylogenetic relationship and closely related taxa among Aeromonas species on the level of 16S rRNA, there were 14 Aeromonas species isolated from poultry farms, 4 from poultry rations (4 A. hydrophila), 2 from poultry water (1 A. hydrophila & 1 A. caviae) and 8 from broiler chickens (2 A. hydrophila & 6 A. caviae). These different isolates of Aeromonas species from poultry farms were investigated and diagnosed by PCR then distinguished by sequence and phylogenetic tree analysis, all selected poultry Aeromonas isolates exhibited the specific amplification of 599 bp fragment. nucleotides sequence of 16S rRNA showed no differences in Selected Poultry Aeromonas isolates of the same source but differed in isolates of different sources (water, ration and broiler), A. hydrophila water was sub-branched and identical to Aeromonas jandaei strain RW102. A. caviae water and A. caviae broiler 6 were closely related to Aeromonas caviae strain T25, A. caviae broiler 1 was identical to Aeromonas veronii strain MS-18, A. caviae broiler 2,3 Were matched to Aeromonas salmonicida subsp. salmonicida 01-B526 and Aeromonas veronii strain 1816, A. caviae broiler 4, 5 were clustered with Aeromonas veronii strain HB18, A. hydrophila ration 1,2 were genetically related to Aeromonas sp. BEG429 while A. hydrophila ration 3,4 were genetically related to Aeromonas sp. BEG034, A. hydrophila broiler 1,2 were genetically related to Aeromonas hydrophila strain FC1361.

Keywords: Aeromonas, PCR sequencing, phylogenetic tree.