European Journal of Biomedical and Pharmaceutical Sciences

REVIEW ON -HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

Gaikwad A. V.*, Devhadrao N. V., Dama G. Y., Bansode A. V., Sale V. and Jadhav P.

ABSTRACT

Chromatography is defined as a set of techniques which is used for the separation of constituents in a mixture. This technique involves 2 phases‟ stationary and mobile phases. The separation of constituents is based on the difference between partition coefficients of the two phases. The chromatography term is derived from the greek words namely chroma (colour) and graphein (to write). The chromatography is very popular technique and it is mostly used analytically. There are different types of chromatographic techniques namely Paper Chromatography, Gas Chromatography, Liquid Chromatography, Thin Layer Chromatography (TLC), Ion exchange Chromatography and lastly High Performance Liquid Chromatography (HPLC). High performance liquid chromatography (HPLC) is a modern application of liquid chromatography. High performance liquid chromatography guarantees a high sensitivity and, at the same time, this technique has its gas analogue. The principle of HPLC is the same as that of liquid chromatography (LC), liquid–solid chromatography (LSC) and liquid–liquid chromatography (LLC). High performance liquid chromatography is the most recent technique. This review mainly focuses on the HPLC technique its principle, types, instrumentation and applications.

Keywords: Ion?Exchange, precipitation, reproducible, HPLC, inositol phosphates.