Abstract
DNA SEQUENCING BY SANGER’S METHOD

*Beena Kumawat

ABSTRACT

The first developed method for DNA sequencing DNA were developed in middle 1970s by Frederick Sanger and Et al. It principally relied on termination of growing nucleotide chain (dNTPs) when a dideoxynucleotide triphosphate (ddNTPs) was inserted in it .Detection of labeled fragments was done by autoradiography on polyacrylamide gel electrophoresis (PAGE). Sanger sequencing given high quality sequences for relatively long stretches of DNA in many disease and genome project.

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