Abstract
VALIDATED UPLC/Q-TOF-MS METHOD FOR SIMULTANEOUS DETERMINATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN HUMAN PLASMA AND ITS APPLICATION TO PHARMACOKINETIC STUDY

Hamid Khan*

ABSTRACT

In the presented work the ultra-performance liquid chromatographic/quadrupole time-of-flight mass spectrometric (UPLC/Q-TOF-MS) method has been developed for simultaneous determination of valsartan and hydrochlorothiazide in human plasma. For identification of drugs, the Q-TOF mass spectrometer was operated in negative ionization mode and quantification was done using the MS/MS transitions at m/z 435.02 to 157.00 for valsartan and 295.80 to 204.94 for hydrochlorothiazide. The chromatographic separation was achieved on Acquity UPLCTM BEH C18 (100.0 × 2.1 mm, 1.7μm) column using isocratic mobile phase consisting of acetonitrile-2mM ammonium acetate (50:50, v/v) at a flow rate of 0.25 mL/min. The calibration curves were linear over the concentration range of 1-1000 ng/mL for all the compounds. The developed method was validated according to ICH guidelines. The method was applied for pharmacokinetic study of valsartan and hydrochlorothiazide in human plasma.

Keywords: UPLC/Q-TOF-MS, Valsartan, Hydrochlorothiazide, Pharmacokinetic Study.


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