European Journal of Biomedical and Pharmaceutical Sciences

IN VITRO ANTIBACTERIAL EFFECTIVENESS TEST OF SEVERAL HERBAL PLANT EXTRACT IN AN ATTEMPT TO DISCOVER THE STRONGEST ANTIBACTERIAL HERBAL TOPICAL AGAINST STAPHYLOCOCUS AUREUS DAN PSEUDOMONAS AERUGINOSAE

Mohamad Andrie and Wintari Taurina*

ABSTRACT

Researchers have tested the activity of the water phase ointment and the cork fish oil extract phase in acute stage II open wounds. The results showed a significant healing potency towards negative controls. However, it does not provide optimal results because a crust was formed. Therefore, researchers used a closed system (dressing) that is able to eliminate crust. Based on the research conducted, crust was not form in the activity test done by researcher using cork fish ointment with male white rats (Rattus norvegicus) wistar strain. But there is a new problem arise such as infection. Based on these results, the researchers aim to look for herbal extracts that can be used as the most powerful antibacterial that will be use in topical wound drug formulation and be tested in vivo to see the effect of wound healing towards white wistar strain rats. This will then be further develop into topical wound drug product. Researchers have conducted in vitro literature studies on several plants, discovered approximately 18 plant extracts that have antibacterial benefits. Antimicrobial activity was measured in vitro by the Kirby Bauer method, which use Mueller-Hinton Agar (MHA) as a bacterial growth medium. There are two bacteria used, namely Staphylococus aureus and Pseudomonas aeruginosa, where each bacterium is tested thrice on the extract of each plant with a bacterial suspense concentration of 1.5 x 108 cells / mL. Positive control using ciprofloxacin injection of 2 mg / mL. Inhibition zone formed is measured using a calipers (caliver). The inhibitory zones produced in each plant extract were then compared with each other. The data obtained will be displayed in the form of descriptive statistics. Sample with largest inhibitory zone respectively are clove oil and green betel extract, with significantly different significance value compared to other plant extract.

Keywords: Antibacterial, topical, herbal plant extract, in vitro.