European Journal of Biomedical and Pharmaceutical Sciences

ACUTE TOXICITY OF METHANOL EXTRACT OF ACORUS SP. RHIZOMES ON THE FEMALE RATS WISTAR STRAIN

Ressi Susanti*

ABSTRACT

Acorus sp. is a traditional medicinal plant that is empirically widely used by the Dayak inland communities in treating various diseases. Scientifically Acorus sp. has been proven to have various activities including anti-bacterial, anti-inflammatory, anti-pain, nephroprotector and hepatoprotector. This study aims to obtain a lethal dose 50 (LD50) from methanol extracts of Acorus sp. rhizomes and observe the effects on behavior, body weight and organ index. The acute toxicity test method was adapted from the OECD 425 guidelines using female wistar rats. The Acorus sp. rhizome was extracted by maceration method using methanol as a solvent. The results of the study indicate that the Acorus sp. rhizome contains flavonoids, phenols, tannins, triterpenoids, saponins and alkaloids. Testing the toxicity of methanolic extract of red Acorus sp. rhizome with a limit test of 2000 mg / kg and 5000 mg / kg, there is no death in experimental animals so that LD50 is greater than 5000 mg / kg. The conclusion of this research is the Acorus sp. rhizome methanol extract is in the category of relatively non-toxic.

Keywords: OECD 425, Acorus sp. rhizome, acute toxicity.