European Journal of Biomedical and Pharmaceutical Sciences

SYNERGISTIC ANTI-ANXIETY ACTIVITY EVALUATION OF DIFFERENT COMPOSITION OF VOLATILE OIL OF EUCALYPTUS AND NEEM OIL BY ELEVATED PLUS MAZE MODEL

Amrita Asthana*, Vandana R. Nair and Kishu Tripathi

ABSTRACT

To evaluate anti-anxiety activity of eucalyptus and neem oils in Swiss albino Mice. In this study six groups (n=5) of mice were used. Standard, control and test drugs were given to each animal orally; behaviour testing was performed in animal models after 30 min time spent in open arm was observed for 5 min duration (300 s). Significant increase in percentage of time spend in open arm of EPM method indicate anxiolytic effect respectively. Significant decrease in above parameters indicates anxiogenic effect. Eucalyptus and neem oil in 1:1, 1:2, 1:3, 2:1 and 3:1 ratios significantly increased percentage of time spend in open arm of EPM method as compared to vehicle treated mice in both EPM behavioural models.

Keywords: Anxiety, Eucalyptus and Neem oil, Elevated plus Maze Model, Dizepam.