ACETYLCHOLINESTERASE INHIBITORY ACTIVITY OF THANEERVITTAN NEI, A NEUROPROTECTIVE SIDDHA FORMULATION USING INVITRO TECHNIQUE
Elakkiyaa V.*, Nisha J., Visweswaran S. and Muthukumar N. J.
ABSTRACT
Neurodegenerative disorders like Alzheimer's disease (AD) is a main cause of dementia, accounting for up to 75% of all dementia cases and has become a population aging-related concern for policymakers and public health systems around the world. Natural products have already proven to be promising sources of useful acetylcholinesterase (AChE) inhibitors. The currently approved drugs for AD, galantamine and rivastigmine are plant derived alkaloids which offer only symptomatic relief without preventing the progression of the disease. In search of identifying new AChE inhibitors preferably from siddha system of traditional medicine which is well tolerated and high efficacious on nervous system. The present study aimed at exploring the potential of siddha formulation ThaneervittanNei (TVN) for its acetylcholinesterase (AChE)enzyme inhibition property by usingEllman’s method. The result obtained from the present clearly indicates that the test drug TVN was effective in inhibiting AChE enzyme at stipulated concentration dose dependently. Maximum percentage inhibition of about 47.25% was observed at 500 μg/ml with the IC 50 value of 503.4±49.17 μg/ml when compare to that of the standard drug physostigmine, a known AChE Inhibitor with the maximum inhibition 86.62% at the concentration of 40 μg/ml with the IC 50 value of 10.85 ± 1.381 μg/ml. It was concluded from the observation of the present study that formulation like TVN has a tendency to inhibit AChE enzyme and may tend to halt the progression of neurodegeneration in near future.
Keywords: Siddha, ThaneervittanNei, Alzheimer's disease, acetylcholinesterase (AChE) inhibitor, Physostigmine.
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