THE EFFECT OF TOTAL PHENOL AND FLAVONOID TOTAL COMPOUNDS OF THE N-BUTANOL AND ETHYL ACETATE FRACTION OF ACORUS SP. TO ANTIBACTERIAL ACTIVITIESIN STREPTOCOCCUS AUREUS AND ESCHERICIA COLI
Nera Umilia Purwanti*
ABSTRACT
Background: Red Jeringau or Acorus sp. is a wild plant that lives in wet and moist soil. The red jeringau rhizome is empirically used by the Dayaks as a treatment for dengue and according to Saman et al. (2013) states that it is traditionally used as a stomach medicine and skin disease. The aims of this study to determine the activity of antibacterial against Staphylococcus aureus and Eschericia coli bacteria from the n-butanol and ethyl acetate fraction of red jeringau rhizomes and the levels of total phenols and total flavonoids of n-butanol fraction and ethyl acetate red rhizome which give antibacterial activity. Method: The study was conducted by measuring total phenol levels and total flavonoids as well as the diameter of inhibition zones of Staphylococcus aureus and Eschericia coli in the n-butanol and ethyl acetate fractions with positive control using ciprofloxacin. The results of inhibition zone diameter data were analyzed using SPPS One Way ANOVA analysis. Result: The results obtained total phenol levels for n-butanol fraction of 10.32% and total flavonoid levels of 0.688% had significant differences with the positive control group at a concentration of 100% (10.44 ± 0.32) against Staphylococcus aureus and at a concentration of 50% (12.14 ± 0.48) and 100% concentration (12.11 ± 0.16) against Eschericia coli. In the ethyl acetate fraction with phenol levels of 43.26% and flavonoid levels of 1.49% the 100% concentration group (12.43 ± 1.29) was significantly different from the positive control group against Eschericia coli bacteria.
Keywords: Red jeringau, total flavonoids, total phenol, antibacterial, n-butanol fraction and ethyl acetate.
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