Abstract
SHOOT INDUCTION FROM TENDRIL EXPLANTS OF LUFFA ACUTANGULA L. A VEGETABLE CROP PLANT

*Mandaloju Venkateshwarlu

ABSTRACT

Shoot induction was achieved in one of the impartant medicinal plant of cucurbitacae family, Luffa acutangula. MS medium supplemented with 1.0 mg/l BAP + 2.0 mg/l NAA and 2.0 mg/l L-Glutamicacid was found to be optimum to induce shoots. The present study established reliable and reproducible protocol for rapid multiple shoot induction from tendril explants of Luffa acutangula using different concentrations and combinations of cytokinins. Tissue culture techniques are now becoming popular as alternative means of vegetative propagation. The effect of benzyl amino purine in inducing shoot induction was already reported in some of the important medicinal plants (Komalavalli and Rao, 2000). The Tendril explants were inoculated on MS basal medium supplemented with various cytokinins i.e., BAP and NAA. Coconut water also had a role in triggering the formation of multiple shoots. Addition of BAP at 2.0 mg/l concentration or NAA at 3.0 mg/l to the MS basal medium, induced regeneration from the Tendril explants.

Keywords: Tendril explants, shoots, L- Glutamic acid, NAA BAP.


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