Abstract
ASSESSMENT OF THE IMPACT OF INHALED TOLUENE IN VAPOUR EXPOSURE CHAMBER ON RENAL AND LIVER FUNCTIONS AND OXIDATIVE STRESS IN RATS

*Olorunfemi O.J., Amah-Tariah F.S. Asara Azibalua A., Banigo Tamunotonye

ABSTRACT

This study assessed the effect of toluene inhalation on kidney and liver functions and markers of oxidative stress in rats. Twenty-four rats were grouped randomly into four groups – each group having six rats. Group 1 (control), group 2 (exposed to toluene vapour at concentration of 8,200 ppm for 14 days), group 3 (exposed to toluene vapour at concentration of 4,100 ppm for 28 days), and group 4 (exposed to toluene vapour at concentration of 2,050 ppm for 56 days). Using a whole-body vapour exposure chamber, animals in groups 2 – 4 were put through toluene vapour exposure challenge. Blood samples from rats in all groups were examined to determine the concentration of urea, creatinine, and electrolytes in serum. Likewise examined were the levels of alkaline phosphatase (ALP), aspartate transaminase (AST), and alanine transaminase (ALT) enzymes, alongside total protein and albumin in serum. The concentration in serum of markers of oxidative stress; malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT)were estimated. Data showed that when compared with the control, in the 14-day group; inhaled toluene significantly increased (p ≤ 0.05) potassium ion concentration, chloride ion concentration, and the level of urea and ALT enzyme. Also, the level of GSH and MDA (11.16±2.66) significantly increased (p ≤ 0.05) while albumin level significantly decreased (p ≤ 0.05). In the 28-day group, chloride ion concentration, urea level and AST enzyme level significantly increased (p ≤ 0.05). Additionally, the level of GSH and MDA (10.59±0.58) significantly increased (p ≤ 0.05) while ALP enzyme level significantly decreased (p ≤ 0.05).In the 56-day group, in comparison with the control, albumin level significantly decreased (p ≤ 0.05). However, no statistically significant change was observed in the concentration of potassium and chloride ions, urea, ALT, AST, and ALP levels, and in the level of GSH and MDA (1.63±0.35). These outcomes thus demonstrate a strong connection between observed concentration and duration dependent toluene vapour toxicity in the kidneys and liver, and the existence of severe oxidative stress-related tissue injury following exposure to toluene vapour.

Keywords: Toluene, Inhalation, Oxidative Stress, Vapour Toxicity, Tissue Injury.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Tangudu Nagabhusana Rao

Download Article : Click here

News & Updation

  • NEW SEPTEMBER ISSUE PUBLISHED

    SEPTEMBER 2020 Issue has been successfully launched on 1 September 2020.

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 5.467 to 6.044, due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT