European Journal of Biomedical and Pharmaceutical Sciences

RESPONSES OF SOME SELECTED ANALGESIC AGENTS TO MECHANICALLY-INDUCED PAIN USING UGO BASILE ANALGESY METER

*Olorunfemi O. J., Amah-Tariah F. S. and Asara Azibalua A.

ABSTRACT

The use of the analgesy-meter in the screening of the analgesic potentials of diclofenac-sodium, piroxicam and paracetamol in rats was investigated by inducing mechanical pain during the assessment in a standard laboratory. Twenty albino Wister rats were placed randomly into four groups of five animals each. Normal saline was given to the control, diclofenac sodium, piroxicam and paracetamol were administered intraperitoneally to the treatment groups at relative doses of (0.05ml/100g, 0.1ml/100g, 0.15ml/100g and 0.2 ml/100g of the body weight of rat). Pain threshold was analysed using the animal response to pain and stimuli and the rotational movement of these animals at the doses where monitored after 0, 30 and 60 mins after administration. The gait pattern was monitored using the Rotometer to observe the animal’s free rotational movement after painful stimuli. Statistical analysis was performed with ANOVA, while Post Hoc multiple comparison test was used in the comparison of the analgesic effects of the control group with the diclofenac treated, piroxicam and paracetamol treated groups with relation to mechanical pain. Results obtained were statistically analyzed and showed that the paw withdrawal threshold was seen to be significantly different (p<0.05) at low to moderate doses of the treatment groups when compared with the control. From the results, it was observed that the intraperitoneal administration of low to moderate doses of the selected analgesics such as the diclofenac sodium, piroxicam and paracetamol produced inhibition to pain when screened using the analgesy-meter and has shown the usefulness and reliability of the analgesy-meter in the screening of the selected analgesic agents.

Keywords: Analgesy meter, Diclofenac, Piroxicam, Paracetamol, analgesic agents, Pain.