European Journal of Biomedical and Pharmaceutical Sciences

INTERFERENCE OF 1, 3, 7-TRIMETHYLXANTHINE (CAFFEINE) INGESTION ON MOTOR BALANCE AND POSTURAL REFLEX

*Olorunfemi O. J., Amah-Tariah F. S. and Asara Azibalua A.

ABSTRACT

The research was carried out to investigate the effect of pure caffeine administration on the postural reflex using healthy wistar rats weighing 180-240g for 3 weeks. A total of 20 rats were divided into four groups. All the rats were given clean water and rat feed throughout the study. The group one animals (control group) were not given any drug, while the animals in group two, three and four were given drugs intra-peritoneally: group two were given 1ml/100g of Celebrex, group three and four were given pure caffeine at concentrations of 0.5ml/100g and 1ml/100g respectively. The effect of caffeine on postural reflex was carried out on both control and test groups, the animals first underwent a mild shock of 3.5mA using an Electroconvulsive therapy, then the spatial learning and memory was assessed with Barnes-maze task, the postural reflex was assessed using Rotarod task and Beam-walk task. The measurement of latency on all the tasks was taken at time range of 5minutes. The results were presented as mean value standard error of Means (±S.E.M). Mean drugs (Celebrex and Caffeine) effect was analyzed using the Analysis of Variance (ANOVA). The findings showed that there was significant increase (P≤0.05) in spatial learning and memory in the animals that were given Celebrex and Caffeine, and there was significant increase (P≤0.05) in the spatial learning and memory in the animals in group four compared to the animals in group one, two and three. It was observed that there was no significant difference (P≥0.05) on the postural reflex on the animals that were given caffeine. From this research it was observed that caffeine may have effect on spatial learning and memory but do not have significant effect (P≥0.05) on the postural reflex.

Keywords: Celebrex and Caffeine.