European Journal of Biomedical and Pharmaceutical Sciences

EVALUATION OF HAEMATOLOGICAL CHANGES IN LEAD-ACETATE-INDUCED ALBINO RATS TREATED WITH AQUEOUS EXTRACT OF HYPOESTES ROSEA LEAF

Uwikor F. K.*, Nwachuku E. O., Igwe F., Echonwere B. and Bartimaeus E. S.

ABSTRACT

Lead is a dangerous heavy metal which is widely spread in the environment and haematological parameters may provide an indication of lead intoxication. This study we assessed the effect of aqueous extract of Hypoestes rosea (AEHR) leaf on the haematological parameters of lead acetate induced male and female albino rats at acute and sub chronic stages in pretreatment and post treatment phases. Animals were divided into 17 groups of five each for both sexes in the treatment groups, while the positive control group had 10 animals in each sex. 8 groups were for the acute phase of the study for 21 days in each sex, while 8 were for 35 days for the sub chronic stage of the study. NC group received rat feed only, EC group received 100mg/kg bwt/day for 21 days at acute and 35 days for sub chronic. PC group received 60mg/kg b.wt per day of lead acetate for 7 days. The other 3 groups received 100mgkg, 200mg/kg and 300mg/kg b.wt respectively for 14 and 28 days either as pre-treatment or post treatment, for both sexes of the albino rats. Samples were taken at the end of the study period through the jugular vein under diethylether anaesthesia, Results showed that the administration of lead acetate on both sexes of the albino rats caused a significant (p<0.05) decrease in the mean HB of the albino rats. The administration of the different doses of AEHR on the albino rats showed a significant (p<0.05) increase in the mean HB of all the groups as compared to the PC group. The result of our study shows that the administration of lead acetate on both sex of the albino rats caused a significant (p<0.05) increase in the WBC count of all the rats. The result of our study shows the administration of varying doses of AEHR on both sexes of the albino rats in the pretreatment and post treatment phase of the acute and chronic stage of the study showed a dose dependent reduction and reversal of the increased total WBC.

Keywords: Haematological Hypoestes rosea Lead-acetate albino rats.