Abstract
IN VITRO EVALUATION OF ANTIMICROBIAL AND BRINE SHRIMP LETHALITY BIOASSAY OF DECOCTION OF AMARANTHUS TRICOLOR

Md. Siddiqul Islam*, Md. Fahad Hossain, Zebunnesa Ahmed and Salma Parvin

ABSTRACT

The decoction of the leaves of Amaranthus tricolor was set for evaluating antimicrobial and cytotoxic activities. With the help of Kanamycin (30μg/disc) used as positive control we evaluated the antimicrobial activity of the decoction by using disk diffusion method. We achieved different degrees of susceptibility of the microorganisms with this decoction. Among 17 different types of microorganisms Bacills sereus, Salmonella paratyphi, Vibrio parahaemolyticus, Salmonella typhi, Candida albicans showed more activity with the extract. Investigation for evaluating cytotoxic activity of the decoction was done by examining the lethality of brine shrimp nauplii. Vincristine sulphate was used as positive control in this experiment. We observed LC50 as 11.02 μg/ml and 0.32 μg/ml from the extract of Amaranthus tricolor and vincristine sulphate repectively. It can be suggested form the results of study that decoction of Amaranthus tricolor may be used for treating different types of bacterial and fungal infections. It further indicates that cytotoxic compound may be searched from the extract of Amaranthus tricolor in future investigations.

Keywords: Amaranthus tricolor, Antimicrobial, Kanamycin, Vincristine.


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