European Journal of Biomedical and Pharmaceutical Sciences

MICROWAVE ASSISTED SYNTHESIS OF SILVER NANOPARTICLE BY USING AQUEOUS ROOT EXTRACT OF OCIMUM SANCTUM AND THEIR ANTIMICROBIAL ACTIVITY

N. H. Bansod*

ABSTRACT

Development of green nanotechnology is creating interest of researcher towards synthesis of nanoparticles. In the present study, microwave assisted synthesis of stable silver nanoparticles by using aqueous root extract of Ocimum sanctum. The biosynthesized nanoparticles were characterized by using UV–Vis, Scanning electron microscopy (SEM), X-ray diffraction (XRD) and FTIR analysis. The observed peaks in XRD pattern corresponding to (111), (200) and (220) planes. The size of the synthesized silver nanoparticles in the range from 36-45 nm. Further Crystallinity of nanoparticle was confirmed by XRD pattern. The FTIR measurement was carried out to identify the possible bio molecules responsible for efficient stabilization of silver nanoparticles. The surface morphology reveals that silver nanoparticles have spherical shape. The antimicrobial activity of silver nanoparticle was screened against gram positive and gram negative microorganisms

Keywords: Silver nanoparticle, ocimum sanctum, antibacterial activity.