ISOLATION, IDENTIFICATION AND COMPARATATIVE STUDY OF SDA AND DTM FOR DERMATOPHYTES FROM CLINICAL SAMPLES IN A TERTIARY CARE HOSPITAL, KANCHIPURAM
Dr. M. Anuradha*, Dr. K. Muthulakshmi and Dr. Somasunder
ABSTRACT
Back ground: The study on dermatophytes based on their morphology by 10-20 %KOH mount, cultural characteristics on various media, Slide culture are useful for their prevention as well as cure as these infections are most common and associate with significant morbidity and disfigurement. Though dermatophytic infections are treated based on clinical manifestations alone, because of the delay in their isolation by using fungal culture media,(7-14 days) t,he clinical manifestation of the infection alone is not useful to identify the causative organism. Thus a study is conducted on isolation, identification and comparison between two fungal isolation media. Materials & methods: 10 -20%KOH, Dermatophyte medium, SDA are used for the study. Results: Among 220 clinically diagnosed cases of superficial mycotic infections 132 cases were dermatophytic infections 88 cases were due to other .66.6% were males and 44.4% were females. commonest infection being Tinea corporis(54.28%) and the commonest species isolated were Trichophyton rubrum.(51.45%).The cases were common during November and December months. In present study cultivation by using DTM was sensitive (76.08%) than SDA medium.(71.73%) and 10 –20% KOH mount (54.34%). Conclusions: By using dermatophyte medium the identification of various dermatophytes can be made accurately and specifically. More over because of incorporating cycloheximide and chloramphenicol the contamination is reduced.
Keywords: dermatophytes, commonest infection, cycloheximide and chloramphenicol.
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